38 Bolton is operated by GO Transit in branches as follows:
| Stop | Fare Zone | Route Number | |
|---|---|---|---|
| 38 | 38A | ||
| Bolton Hwy. 50 @ Columbia Way |
58 | • | |
| Bolton Albion-Vaughan @ Industrial |
• | ||
| Bolton Queen @ Allan |
• | • | |
| Nobleton King @ Hwy. 27 |
57 | • | |
| Bolton South P&R Hwy. 50 @ Mayfield |
56 | • | |
| Woodbridge Hwy. 50 @ Hwy. 7 |
• | ||
| Kleinburg Islington @ Nashville |
• | ||
| Woodbridge Rutherford @ Vaughan Mills |
• | ||
| Malton GO Derry & Airport |
31 | • | |
| Toronto Hwy. 27 @ Humber College |
4 | • | |
| Etobicoke North GO Belfield & Kipling |
• | ||
| Yorkdale Terminal Subway - Métro |
5 | • | |
| York Mills Terminal Subway - Métro |
• | ||
38[]
Southbound to Malton GO[]
From Bolton (Hwy. 50 @ Columbia Way), the bus travels:
- south 3.1 km on Queen Street to Allan Drive, making a stop at Bolton (Queen @ Allan)
- south 0.9 km on Queen Street to Highway 50
- continuing south 2.8 km on Highway 50 to Mayfield Road
- west 0.3 km on Mayfield Road, making a stop at Bolton South P&R
- south 9.7 km on Highway 50 to Highway 7, making a stop at Woodbridge (Hwy. 50 @ Hwy. 7)
- continuing south 2.1 km on Highway 50 to Steeles Avenue
- west 1.0 km on Steeles Avenue to Finch Avenue
- east 1.8 km on Finch Avenue to Highway 427
- south 2.0 km on Highway 427 to Derry Road
- west 1.9 km on Derry Road, terminating at Malton GO
Northbound to Bolton (Hwy. 50 @ Columbia Way)[]
From Malton GO, the bus travels:
- east 2.1 km on Derry Road to Highway 427
- north 2.3 km on Highway 427 to Finch Avenue
- west 2.2 km on Finch Avenue to Steeles Avenue
- east 1.0 km on Steeles Avenue to Highway 50
- north 2.1 km on Highway 50 to Highway 7, making a stop at Woodbridge (Hwy. 50 @ Hwy. 7)
- continuing north 9.8 km on Highway 50 to Mayfield Road
- west 0.3 km on Mayfield Road, making a stop at Bolton South P&R
- east 0.3 km on Mayfield Road to Highway 50
- north 2.8 km on Highway 50 to Queen Street
- continuing north 0.9 km on Queen Street to Allan Drive, making a stop at Bolton (Queen @ Allan)
- continuing north 3.1 km on Queen Street to Columbia Way, terminating at Bolton (Hwy. 50 @ Columbia Way)
38A[]
Southbound to York Mills Terminal[]
From Bolton (Albion-Vaughan @ Industrial), the bus travels:
- north 1.8 km on Albion-Vaughan Road to Queensgate Boulevard
- west 1.2 km on Queensgate Boulevard to Queen Street
- north 0.5 km on Queen Street to Allan Drive, making a stop at Bolton (Queen @ Allan)
- continuing north 1.2 km on Queen Street to King Street
- east 2.0 km on King Street to Caledon-King Townline
- continuing east 5.5 km on King Road to Highway 27, making a stop at Nobleton (King @ Hwy. 27)
- south 6.3 km on Highway 27 to Islington Avenue
- south 0.7 km on Islington Avenue to Nashville Road, making a stop at Kleinburg (Islington @ Nashville)
- continuing south 3.8 km on Islington Avenue to Rutherford Road
- west 1.4 km on Rutherford Road to Vaughan Mills Road, making a stop at Woodbridge (Rutherford @ Vaughan Mills)
- continuing west 1.1 km on Rutherford Road to Highway 27
- south 9.3 km on Highway 27 to Humber College Boulevard, making a stop at Toronto (Hwy. 27 @ Humber College)
- continuing south 3.7 km on Highway 27 to Belfield Road
- east 1.7 km on Belfield Road, making a stop at Etobicoke North GO
- continuing east 0.3 km on Belfield Road to Kipling Avenue
- south 0.1 km on Kipling Avenue to Highway 409
- east 1.1 km on Highway 409 to Highway 401
- east 8.6 km on Highway 401 to Yorkdale Road, exit 364
- east 0.8 km on Yorkdale Road, making a stop at Yorkdale Terminal
- east 4.9 km on Highway 401 to Yonge Street, exit 369
- south 0.5 km on Yonge Street, terminating at York Mills Terminal
Northbound to Bolton (Albion-Vaughan @ Industrial)[]
From York Mills Terminal, the bus travels:
- north 1.0 km on Yonge Street to Highway 401
- west 5.2 km on Highway 401 to Yorkdale Road, exit 365
- south 0.3 km on Yorkdale Road, making a stop at Yorkdale Terminal
- west 8.9 km on Highway 401 to Highway 409, exit 355
- west 1.9 km on Highway 409 to Belfield Road
- west 0.3 km on Belfield Road, making a stop at Etobicoke North GO
- continuing west 1.7 km on Belfield Road to Highway 27
- north 3.7 km on Highway 27 to Humber College Boulevard, making a stop at Toronto (Hwy. 27 @ Humber College)
- continuing north 9.3 km on Highway 27 to Rutherford Road
- east 1.1 km on Rutherford Road to Vaughan Mills Road, making a stop at Woodbridge (Rutherford @ Vaughan Mills)
- continuing east 1.4 km on Rutherford Road to Islington Avenue
- north 3.9 km on Islington Avenue to Nashville Road, making a stop at Kleinburg (Islington @ Nashville)
- continuing north 0.6 km on Islington Avenue to Highway 27
- north 6.3 km on Highway 27 to King Road, making a stop at Nobleton (King @ Hwy. 27)
- west 5.5 km on King Road to Caledon-King Townline
- continuing west 2.0 km on King Street to Queen Street
- south 1.2 km on Queen Street to Allan Drive, making a stop at Bolton (Queen @ Allan)
- continuing south 0.5 km on Queen Street to Queensgate Boulevard
- east 1.3 km on Queensgate Boulevard to Albion-Vaughan Road
- south 1.8 km on Albion-Vaughan Road to Industrial Road, terminating at Bolton (Albion-Vaughan @ Industrial)